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Friends of the Earth conducted this peer-reviewed study in collaboration with researchers at University of California at Berkeley, University of California at San Francisco, and the Commonweal Biomonitoring Resources Center. We wanted to know whether eating an organic diet could reduce levels of detectable pesticides and pesticide breakdown products in participants’ bodies.

We secured oversight for protection of human subjects through Western Institutional Review Board. The study was published in the journal Environmental Research.

We chose four families in Atlanta, Baltimore, Minneapolis and Oakland to participate in the study. These families reported that they did not typically eat an organic diet. In total, 16 people participated in the study.

The study lasted 12 days. During the first phase, each family ate only conventional (non-organic) foods and beverages. During the second phase, each family ate only organic foods and beverages. Each participant collected a daily urine sample during the 12 days of the study and kept a food diary.

To ensure that the families were able to eat a completely organic diet, organic food was provided to them in two ways: 1) participants were asked to compile a list of all groceries they would need for a week, and research assistants purchased organic foods from this list and delivered the groceries to the participants’ homes; and 2) dinners during the organic week were prepared with all organic foods by a licensed chef or caterer and delivered to study participants by the research assistants. All organic foods were provided free of charge to the families.

The samples were analyzed for by laboratories at the University of California at San Francisco and the Québec National Institute of Public Health.

For a full discussion of methods, see Organic Diet Intervention Significantly Reduces Urinary Pesticide Levels in U.S. Children and Adults in Environmental Research, a peer-reviewed scientific journal.

University of California at San Francisco Lab Methodology

Urine samples were analyzed for 18 pesticide analytes, including nine specific organophosphate, neonicotinoid, pyrethroid, fungicide and herbicide analytes and three non-specific pyrethroid analytes.

Quantification of the nine pesticide-specific analytes and three non-specific pyrethroid analytes was performed using liquid-chromatography-tandem mass spectrometry (LC-MS/MS) on an Agilent LC 1260-AB Sciex 5500 system. The urine specimens (1 mL) were prepared for LC-MS/MS analysis by solid phase extraction (SPE) using Waters Oasis WAX cartridges (10mg, 30um, 1cc). The compounds were ionized in the negative mode using electrospray ionization (ESI) and monitored by multiple reaction monitoring. Each compound was monitored using two transitions (see Supplementary Information S1) along with 2,4-D-d3 and Cloth-d3 as internal standards). Each batch of samples was injected in duplicates and run alongside calibration standards that were run at the beginning, between the duplicate sample injections and after the sample set. Additionally, low and high spiked quality control (QC) samples were run for each analysis batch. A batch run was accepted if both QC samples were within 20 percent of their target values and had coefficients of variation (CV) ≤ 20 percent.

Québec National Institute of Public Health

Urine samples were analyzed for six non-specific OP dialkyl phosphate (DAPS) analytes.

To quantify the six non-specific OP pesticide DAP metabolites (DEP, DETP, DEDTP, DMP, DMTP and DMDTP), 0.1 mL of the urine specimens were enriched with internal standards (DEP-13C4, DETP-13C4, DEDTP-13C4, DMP-d6, DMTP-d6, DMDTP-d6). After adding 1 mL of acetonitrile and 200 mg of potassium carbonate, the samples were derivatized with 15 µL of pentafluorobenzyl bromide (PFBBr) at 70°C for 2 hours. The derivatized products were extracted with 7 mL of a mixture of dichloromethane:hexane (8:92), mixed 15 minutes and centrifuged 5 minutes at 1500 rpm. The solvent was then evaporated to dryness, taken up in 500 µL of dichloromethane:hexane (20:80) and analyzed for pesticide metabolites on an Agilent 6890 Network gas chromatograph (GC) (Agilent Technologies; Mississauga, Ontario, Canada) coupled to a Waters Quattro Micro GC mass spectrometer in tandem (MS/MS) (Waters; Milford, Massachusetts). The GC was fitted with an Agilent 30 m HP-5MS column (0.25 mm i.d., 0.25 µm film thickness) to the MS/MS. The internal reference materials used to control the quality of the analyses were the non-certified reference material ClinChek (Urine Level 1; RECIPE Chemicals; Munich, Germany) and three in-house reference materials (low, medium, high QC) prepared by the Centre de Toxicologie du Québec (CTQ), Institut National de Santé Publique du Québec (INSPQ). The overall quality and accuracy of the analytical method was monitored by the interlaboratory program as the German External Quality Assessment Scheme (G-EQUAS; Erlangen, Germany).

The labs tested for the following chemicals and did not detect them in any participant urine samples: 5-Hydroxy-Imidacloprid, 5-OH-Thiabendazole (a breakdown product of thiabendazole, used in agriculture as a fungicide), Iprodione (a fungicide and nematicide), and Boscalid (a fungicide). This could mean that participants were not exposed to these chemicals. However, all of the pesticides these chemicals represent are used in significant quantities and are frequently found as residues on food (see USDA Pesticide Data Program.) Because the science of studying pesticides in people’s bodies is still evolving, it could also mean that the methods the labs used were not sensitive enough to detect these chemicals or that the specific chemicals studied are not the correct compound to look for. For example, this study found the neonicotinoid pesticide clothianidin in every participants’ urine but did not find the neonicotinoid breakdown product for imidacloprid (5-Hydroxy-Imidacloprid) despite the fact that imidacloprid is far more widely used in agriculture.